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. 2016 Jun 23;17:471. doi: 10.1186/s12864-016-2815-y

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Conceptual graph of single cell genomics workflow applied to Cryptosporidium parvum. a Scattergram of side scatter and antigen-based fluorescence used for sorting individual cells. The gate for sorting is indicated by a circle and individual analyzed cells are marked by color. Panel with antibody stained oocysts to the left. b. Real-time MDA kinetics for individual amplified genomes color coded as in (a). Blanks are given in brown. c Small subunit rRNAscreening of amplified cells with gel electrophoreses and sequencing. Negative control and positive control with 10 cells are included for comparison