Table 1. Import characteristics for recombinant H. pylori clones from transformation cycle and short-term transformation experiments.
| 26695 transformed with J99-R3Cm gDNA (20 clones) | 26695 transformed with J99-R3ΔGATC gDNA (12 clones) | 26695 transformed with J99-R3ΔGANTC gDNA (6 clones) | |
|---|---|---|---|
| Total no. of CNPs/SPIs | 826 | 547 | 224 |
| Mean no. of CNPs/SPIs per strain | 41.3±24.1 | 45.6±25.9 | 37.3±26.7 |
| Range of CNPs/SPIs per strain | 4–92 | 1–92 | 1–65 |
| Mean total combined length of CNPs/SPIs (minimal) (bp) | 55,644.2±36,108.3 | 58,951.3±34,193.0 | 56,010.3±38,819.9 |
| Range of total combined lengths of CNPs/SPIs (minimal) (bp) | 6,249–128,796 | 80–116,126 | 555–108,784 |
| Mean per cent of genome exchanged (minimal) (%) | 3.3±2.2 | 3.5±2.1 | 3.3±2.3 |
| Range of per cent of genome exchanged (minimal) (%) | 0.4–8 | 0.004–6.9 | 0.03–6.5 |
| Mean length of CNPs/SPIs (minimal) (bp) | 1,347.3±1,586.9 | 1,293.3±1,370.2 | 1,500.3±1,624.3 |
| Range of lengths of CNPs/SPIs (minimal) (bp) | 1–13,408 | 1–12,177 | 1–10,143 |
| Median length of CNPs/SPIs (minimal) (bp) | 901.0 | 964.0 | 1,007.5 |
| Mean total combined length of CNPs/SPIs (maximal) (bp) | 60,226.7±38,158.7 | 63,840.3±37,022.9 | 60,210.5±41,712.0 |
| Mean per cent of genome exchanged (maximal) (%) | 3.6±2.3 | 3.8±2.2 | 3.6±2.5 |
| Mean length of CNPs/SPIs (maximal) (bp) | 1,439.4±1,599.6 | 1,400.5±1,373.8 | 1,612.8±1,624.8 |
| Median length of CNPs/SPIs (maximal) (bp) | 1,003.0 | 1,077.0 | 1,139.0 |
| 26695 transformed with J99-R3Cm gDNA (21 clones) | Selected | Non-selected | Combined |
|---|---|---|---|
| Total no. of CNPs/SPIs | 21 | 80 | 101 |
| Mean no. of CNPs/SPIs per strain | 1 | 4.7±4.5 | 4.8±4.5 |
| Range of CNPs/SPIs per strain | ND | 0–20 | 1–21 |
| Mean total combined length of CNPs/SPIs (minimal) (bp) | ND | 4,686.1±4,434.8 | 6,716.3±5,207.6 |
| Range of total combined lengths of CNPs/SPIs (minimal) (bp) | ND | 70–22,297 | 1680–23,777 |
| Mean length of CNPs/SPIs (minimal) (bp) | 2,990.9±1,788.9 | 995.8±1,158.8 | 1,410.6±1,537.2 |
| Range of lengths of CNPs/SPIs (minimal) (bp) | 638–7,601 | 1–5,506 | 1–7,601 |
| Median length of CNPs/SPIs (minimal) (bp) | 2,647.0 | 632.5 | 934.0 |
| Mean total combined length of CNPs/SPIs (maximal) (bp) | ND | 5,136.2±4,777.3 | 7,272.1±5,528.1 |
| Mean length of CNPs/SPIs (maximal) (bp) | 3,195.2±1,625.6 | 1,090.5±1,175.1 | 1,528.1±1,543.6 |
| Median length of CNPs/SPIs (maximal) (bp) | 2,881.0 | 709.5 | 1,098.0 |
CNP, clusters of nucleotide polymorphism; gDNA, genomic DNA; ND, not determined; SPI, single-polymorphism import.