Table 1.
Real-time quantitative PCR: Cell types, primers and results
| Cell type | HTR2B primers | Reference gene primer | HTR2B expression |
|---|---|---|---|
| Human PBMCs | Taqman primer Hs00168362_m1 | GAPDH: Taqman primer Hs02758991_g1 | Very low or absent |
| (see Methods) | |||
| Hprt1: Taqman primer Hs02800695_m1 | |||
| ActB: Taqman primer Hs1060665_g1 | |||
| RPLP0: Taqman primer Hs004189669_g1 | |||
| Human T cell populations: | Taqman primer Hs00168362_m1 | GAPDH: Taqman primer Hs02758991_g1 | Very low or absent in all T cell subpopulations |
| -CD3+ | |||
| -CD4+ | |||
| -CD8+ | |||
| -CD4+ CD25+ | |||
| (total RNA obtained from Miltenyi Biotec; Cat. nos: 130-093-165, 164, 163 and 168) | |||
| Human primary monocytes (see Methods) | Taqman primer Hs00168362_m1 | GAPDH: Taqman primer Hs02758991_g1 | Expressed |
| Hprt1: Taqman primer Hs02800695_m1 | |||
| Human immature dendritic cells (total RNA obtained from 3H Biomedical; Cat. no: 3H100-70-5) | Taqman primer Hs00168362_m1 | GAPDH: Taqman primer Hs02758991_g1 | Expressed |
| Hprt1: Taqman primer Hs02800695_m1 | |||
| ActB: Taqman primer Hs1060665_g1 | |||
| RPLP0: Taqman primer Hs00420895_gH | |||
| Human THP-1 monocytes and macrophages (phorbol 12-myristate 13-acetate-differentiated) | Taqman primer Hs00168362_m1 | GAPDH: Taqman primer Hs02758991_g1 | Expressed under inflammatory conditions |
| Hprt1: Taqman primer Hs02800695_m1 | |||
| ActB: Taqman primer Hs1060665_g1 | |||
| RPLP0: Taqman primer Hs00420895_gH | |||
| Mouse peritoneal macrophages (thioglycolate-elicited, see Methods) | Taqman primer Mm00434123_m1 | GAPDH: Taqman primer Mm99999915_g1 | Expressed |
| Hprt1: Taqman primer Mm00446968_m1 |