Figure 5.

PD166326 blocks recruitment of Nck and N-WASP. (a–h) Images of 3T3 cells treated with DMSO (a–d) or PD166326 (e–h) and concomitantly exposed to EPEC. Cells were stained with DAPI (a and e) to recognize EPEC, with FITC-phalloidin to recognize actin (b and f), and with α-Nck mAb (c and g). (i–p) Images of 3T3 cells treated as in a–h and stained with DAPI (I and m), FITC-phalloidin (j and n), and α-N-WASP pAb (k and o). Note that Nck and N-WASP localize at the tip of actin pedestals and that PD blocks Nck and N-WASP localization. Bars, 5 μm.