Figure 3.

RCP interacts with Rab11 but not Rab4 in vivo. HeLa cells were transfected with ether RCP-YFP/CFP-Rab11A (A–D) or RCP-YFP/CFP-Rab4A (E–H), fixed and imaged. cFRET images were generated as described in MATERIALS AND METHODS. NFRET was calculated using the equation NFRET = cFRET/CFP and is an average from at least five randomly picked cells. The yellow signal in C and G represents the overlap between CFP and YFP. In I–H, HeLa cells were transfected with either myc-tagged Rab11-S25N (I and J) or myc-tagged Rab4-I121N (G and H) and costained with anti-myc (I and G) and anti-RCP (J and H) antibodies. Bars, 2 μm.