Figure 5.

RCP recruitment to endosomes is dependent on binding to Rab11. HeLa cells were incubated in the presence (A–C, E–F) or absence (D) of digitonin. Cells were then washed and incubated with either GST (C) or GST-RCP-F1 (A and B, D–F) in the presence (E and F) or absence (A–D) of soluble recombinant Rab11. Cells were fixed and stained with anti-GST (B and F, green; and C and D, gray) and anti-TfR (A and B and E and F, red) antibodies. Yellow in B and F represents the degree of overlap. Bars, 3 μm.