Figure 1.

Internalization of CD59, MHCI, and transferrin in untransfected cells. HeLa cells were incubated at 37°C as described in MATERIALS AND METHODS with FITC-anti-CD59 (green), Cy3-anti MHCI (red), and 5 μg/ml Alexa 633-transferrin (blue) for 2 min and 30 min. PM-associated ligands were removed by low pH treatment before fixation. Inset shows endosomes containing CD59 and MHCI (yellow) at 2 min and endosomes containing CD59, MHCI and transferrin (white) at 30 min. Some endosomes at 2 min only contain CD59 (arrows). Bar, 10 μm.