Figure 6.

CD59 and cholesterol accumulate in Arf6-Q67L-associated vacuolar endosomes, devoid of caveolin-1. (A) Cells expressing Arf6-Q67L were incubated with anti-CD59 at 37°C for 1 h, and surface antibody was removed and then processed for immunofluorescence. After fixation, free cholesterol was stained with filipin as described in MATERIALS AND METHODS. Note the redistribution and intensity of filipin in the transfected cells (encircled). (B) Cells expressing Arf6-Q67L were labeled with antibody to caveolin-1. Bar, 10 μm.