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CD59 and cholesterol reach Rab5 compartment via Arf6-endosomes. HeLa cells were transfected with GFP-Rab5-Q79L alone (A and C) or with GFP-Rab5-Q79L and Arf6-Q67L (B and D). In A and B, cells were allowed to internalize phycoerythrin anti-CD59 antibody at 37°C for 1 h. In C and D, cells were fixed and stained with filipin. Bar, 10 μm.
