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Arginine343 and arginine344 are required for function of Atg21. (A) atg21Δ cells (PSY7) expressing GFP-Atg21 (pPS123) but not GFP-Atg21R343,344K (pPS125) mature prApe1. (B) GFP-Atg21R343,344K does not localize to the vacuole and punctate structures. DIC, differential interference contrast.
