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. 2004 Aug;15(8):3580–3590. doi: 10.1091/mbc.E04-03-0236

Figure 1.

Figure 1.

Attachment and spreading of ECs on NG2. (A) NG2 binding to the EC surface. NG2 immunoreactivity is seen on the surfaces of MAEC incubated with soluble NG2/EC+, but not on untreated cells (control). DAPI was used as a nuclear counterstain. Scale bar, 20 μm. (B) EC attachment to NG2-coated surfaces. MAEC were tested for their ability to adhere to wells coated with Matrigel (30 μg/ml), type I collagen (30 μg/ml), and various concentrations of NG2/EC+ (1–100 μg/ml). Three high-power microscope fields were counted in each replicate well, and results were expressed as cells per field. Each bar represents the mean ± SD (n = 3). Statistically significant differences compared with the control are indicated (*p < 0.005; **p < 0.05). Insets show the stained cells in control (left) and NG2-coated (100 μg/ml) wells (right). Adherent cells in the right-hand panel represent roughly 90% of the input cells. Scale bar, 50 μm. (C) Spreading of ECs on NG2-coated surfaces. Staining with rhodamine-phalloidin was used to visualize F-actin organization in MAEC after adherence for 1 h to wells coated with BSA (control), type I collagen (30 μg/ml), NG2/EC+ (10 μg/ml), or NG2/EC- (10 μg/ml). Scale bar, 20 μm.