Figure 4.

Corneal angiogenesis. (A) Hydron pellets containing PBS (control) or NG2/EC⁺ (500 ng) were implanted into Sprague-Dawley rat corneas. Five days later, vessels growing toward the pellet implant from the limbus were photographed. (B) Rat and mouse corneal angiogenesis in summary. Pellets containing 200 ng of NG2/EC⁺ or 50 ng of bFGF were implanted in the case of BALB/c and C57Bl/6 mice, and 500 or 100 ng, respectively, were used for Sprague-Dawley rats. For antibody inhibition, anti-NG2 mAb or isotype-matched control IgG (2 μg/pellet) was directly added to the pellet before implantation into corneas of C57Bl/6 mice. “Mock purified” material was also tested in C57Bl/6. n.d., not determined.