Figure 6.

Inhibition of EC migration by antigalectin-3 antibody. (A) Detection of galectin-3 on the surface of ECs. Immunofluorescence (left panels) and flow cytometry (right panels) were performed on unpermeabilized MAEC (top panels) and HMVEC (bottom panels) using antigalectin-3 antibody. Scale bar, 20 μm. (B) MAEC were preincubated with either nonimmune rat IgG, anti-CD44, or antigalectin-3 antibody (15 μg/ml) for 15 min and assayed for cell motility in Transwells coated with Matrigel (30 μg/ml), type I collagen (30 μg/ml), fibronectin (10 μg/ml), or NG2/EC⁺ (10 μg/ml). Each bar represents the mean ± SD (n = 3). A statistically significant difference was obtained for antigalectin-3 on NG2/EC-coated Transwells compared with the value obtained in the presence of control IgG (^*p < 0.02).