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. 2004 Aug;15(8):3580–3590. doi: 10.1091/mbc.E04-03-0236

Figure 8.

Figure 8.

EC response to NG2 involves both galectin-3 and α3β1 integrin. (A) HMVEC preincubated for 15 min with either nonimmune IgG, anti-α3, or antigalectin-3 antibody (15 μg/ml) were assayed for motility in Transwells coated with Matrigel (30 μg/ml) or NG2/EC+ (50 μg/ml). Each bar represents the mean ± SD (n = 3). *p < 0.02 indicates statistically significant differences from the value obtained in the presence of control IgG, and **p < 0.05 from the value obtained in the presence of either α3 or galectin-3 antibody alone. (B) Detergent extracts of MAEC were immunoprecipitated with anti-β1 integrin antibody or control IgG. Immunoprecipitates were fractionated by SDS-PAGE and immunoblotted with antibodies against α3 integrin, β1 integrin, and galectin-3. Crude MAEC lysate was also loaded as a positive control. (C) Detergent extracts of A375 cells were immunoprecipitated with anti-NG2 antibody, anti-β1 integrin antibody or control IgG. Immunoprecipitates were fractionated by SDS-PAGE, and immunoblotted with antibodies against NG2, α3 integrin, β1 integrin, and galectin-3. NCAM was immunoblotted as a negative control to show the specificity of the immunoprecipitations.