Fig. 6.

Retrozymes in the genome of different citrus trees. a Northern blot analysis of RNA extracts of C. clementina ovaries, flowers and leaves carried out as described in Fig. 3. 1 pg of a (+)RNA transcript of C. sinensis was included. b Northern blot analysis of RNA extracts of C. limon leaves, flowers and seeds carried out as described in Fig. 3. 0.1 ng of a (+)RNA transcript of C. sinensis was included. The positions of the linear and circular RNAs are indicated. Ethidium bromide staining of the 5S rRNA band is shown at the bottom of panels a and b as a loading control. c Minimum free energy secondary structure prediction for a retrozyme RNA of 689 nt derived from a genomic retrozyme of Citrus clementina (entry NW_006262201). Numbering and colors are shown as in Fig. 3