Table 1.

Colocalization of EGFR, TfR, and epsin in coated pits

	A. TfR and EGFR or EGF label
	EGFR+	EGFR–	EGF+	EGF–
TfR+	15	66	14	54
TfR–	19	—	32	—

	B. Epsin and EGF or TfR
	EGF+	EGF–	TfR+	TfR–
Epsin+	23	46	42	36
Epsin–	31	—	22	—

Thawed cryosections of Hep2 cells, incubated with EGF on ice for 60 min, were double-labeled either for TfR and the EGFR or EGF (A), or for epsin and EGF or the TfR (B). The number of coated pits showing labeling for at least one of the molecules were counted, and the degree of colocalization was calculated. The results are shown as % of the total number of labeled coated pits in each double-labeling.