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Comparison of phototropin levels in flagella between wild type (CC-124) and a strain with reduced phototropin levels (RNAi20). The flagella from strains CC-124 and RNAi20 (Huang and Beck, 2003) were separated by the pH shock method. Flagellar protein (7 μg) was separated by SDS-PAGE (9% polyacryamide) and immunoblotted using a phototropin-specific antibody. For a loading control, an antibody that reacts with the kinesin-II motor protein FLA10 was used.
