Table 1.

CT-induced change in morphology

Condition	% Affected cells (n = 150–250, ±5%)
Single Interference
Eps15Δ95-295	98.5
Cav1S80E	97.1
Arf6Q67L	98.4
Arf6T27N	93.9
Multiple Interferences
Arf6Q67L/Cav1S80E	88
Eps15Δ95-295/Arf6Q67L	96
Dyn1K44A	85
Eps15Δ95-295/Cav1S80E	96.4
Arf6Q67L/Dyn2K44A	76.8

In each case, the concentration of CT used (of EC95 200 pM) was such that even partial inhibition of endocytic entry should result in a detectable effect on toxicity. In addition, overexpression of two other dominant negative mutants of caveolin or preincubation with genistein did not protect Y-1 cells against CT-induced cell shape changes (Fig. 6A and B). Representative fluorescence (displayed in inverted monochrome scale) and phase contrast images of these conditions are shown in Fig. 7 (Supplement).