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. 2004 Aug;15(8):3642–3657. doi: 10.1091/mbc.E03-11-0796

Figure 8.

Figure 8.

COS cells expressing Pc1618–1810 undergo apoptosis during the G2/M transition. (A) Cells expressing 1618–1810 undergo apoptosis at mitosis. Microinjected cells released from a double thymidine block were stained for DNA (DAPI) and overexpressed protein (250 cells/bar). 1618–1810–expressing cells were apoptotic or detached 8–10 h after injection, whereas control cells (Pc B1826–2117) increased in number. At 14 h, p value comparing the two treatments, p < 0.0001 (B) Mitotic index of COS cells after release from double thymidine block as in A (peak, 9 h). (C) 1618–1810–expressing cells arrested in S phase do not undergo apoptosis. Microinjected cells retained in thymidine for the times indicated. No loss of cells was observed 8–14 h later. 10*, microinjected cells arrested in S phase for 6 h and then released from the block for 4 h (10 h total, p = 0.25). (D) Immunofluorescence image of an apoptotic cell expressing 1618–1810 at 10 h postinjection as in A. D (overlay), DNA (blue and upper inset), M30 (red), overexpressed protein (lower inset).