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Actin and microtubule localization. Wild-type (GR5; A, B, and E) and mesA1 mutant (ACP21; C, D, and F) hyphae were grown for 12 h. They were subsequently fixed and processed for immunofluorescence microscopy to detect actin (A–D) or microtubules (E and F). Red arrows, actin rings at septation sites. White arrows, actin patch at hyphal tips; white arrowheads, cytoplasmic microtubules. Bars, 6 μm.
