Figure 2.
Persistent desensitization of the GLP-2 receptor is not species or cell type specific. DLD-1:hGLP-2R cells (A) or BHK cells stably transfected with the rGLP-2R (BHK:rGLP-2R) (B) were incubated with either 10 nM human (A) GLP-2, 10 nM rat (B) GLP-2, or vehicle (control, C) for 20 min; allowed to recover for the indicated periods of time; and then rechallenged with 100 nM GLP-2 or media alone (basal) for 10 min to determine maximal agonist-induced cAMPi accumulation. Data (means ± SD from triplicate values) were normalized and compared with control (C) (*p < 0.05, **p < 0.01, ***p < 0.001) as described in Figure 1 and are representative of two to four independent experiments.