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. 2004 Aug;15(8):3688–3697. doi: 10.1091/mbc.E04-04-0278

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Figure 9. — Flow cytometric analysis and purification of fluorescent endocytic vesicles. Alexa 488–labeled endocytic vesicles were prepared and sorted on a Dykocytomation (MoFLo) Cell Sorter. Regions indicated by lower case letters in circles in this figure correspond to those indicated schematically in Figure 1. (A) Dot plot representation of forward scatter (FSC) vs. pulse width of presorted vesicles. (B) Dot plot representation of FSC vs. side scatter (SSC) of vesicles within region (c) of dot plot (B). (C) and (D) Fluorescent profiles of unlabeled (control) or early (C) or late (D) endocytic vesicles within region (f) of dot plot (B).