Figure 5.

Overexpressed Numb and EHD4 colocalize in HeLa cells. HeLa cells were transfected with pEF-Numb p66 and GFP-EHD4 and fixed 24 h after transfection, and processed for immunofluorescent localization of Numb (red) and GFP-EHD4 (green). Boxed regions have been digitally enlarged and represented as inserts in order to show more clearly examples of colabeling (arrows). In this representative section, Zeiss colocalization software compatible with Zeiss Axiovert 100 with LSM510 estimates there is ∼51% colocalization of Numb and EHD4. Scale bar, 10 μm.