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. 2004 Aug;15(8):3698–3708. doi: 10.1091/mbc.E04-01-0026

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Figure 8. — Activation of Arf6 alters Numb subcellular localization. HeLa cells cotransfected with pEF-Numb p66, GFP-EHD4, and Arf6-Q67L-HA were fixed, permeabilized, and processed for immunofluorescence. Expression of Arf6-Q67L-HA (blue) induces localization of Numb (red) and EHD4 (green) to Arf6-positive membrane protrusions (top panels) and macropinosomes/vacuolar clusters (bottom panels). Boxed regions have been digitally enlarged and represented as inserts in order to show more clearly examples of colabeling. Scale bar, 10 μm.