Figure 1.

Identification of the shortest telomeres. BJ metaphases at PD84 were serially hybridized to both C- and G-rich telomeric probes to maximize the detection of small telomeric regions. (A) Telomeres so short that they failed to give an observable signal (signal-free ends) were identified by CGH analysis. (B) The frequency of signal-free ends per 100 metaphases is shown for all telomeric ends from an analysis of 403 metaphases. BACs within 100 kb of the ends of the telomeres indicated by vertical arrows were used in the analysis shown in Figure 2.