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. 2004 Aug;15(8):3719–3728. doi: 10.1091/mbc.E04-03-0237

Figure 4.

Figure 4.

Nucleosome positioning on the 14-kb rDNA. MNase digestion products from macronuclei or deproteinized DNA were digested with HindIII, and the subtelomeric restriction fragments were identified by Southern hybridization by using a probe that hybridized adjacent to the restriction site (A and B), fragments protected by the telomeric complex were identified using a probe that hybridized 40 nt internal to the telomeric tract (C). The cartoons show the relative positions of the HindIII site (arrow), MNase hypersensitivity (arrowheads), hybridization probes (bar), telomeric complex on days 1 and 5 (overlapping ovals), and nucleosomes (circles). (A) Digestion products from the native rDNA telomere after 1 or 5 d culture at 30°C. Left, macronuclei were digested with 30 U of MNase/mg chromatin for 0, 2, 4, 6, 16, or 60 min. Right, deproteinized DNA controls; digested with 8 U of MNase/mg DNA for 1 or 2 min. (B) Same blot hybridized with the probe to the recombinant subtelomeric sequence. (C) Day 1 blot from A hybridized with probes 40 base pairs internal to the telomeric complex from the native telomere (right) or the recombinant telomere (left).