Table 1.
Velocities of unc-104(e1265) animals or unc-104 animals expressing wt UNC-104 or UNC-104 motors bearing the indicated deletions, PH domain substitutions, or PH domain point mutations
| Construct | Velocity (mm/min) | SD | n |
|---|---|---|---|
| unc-104(e1265) | 1.1 | 0.9 | 150 |
| wt | 12.7 | 3.6 | 454 |
| ΔPH | 0.9 | 1.0 | 772 |
| CAAL | 2.3 | 1.6 | 268 |
| Δ878–1339 | 2.4 | 0.7 | 313 |
| Δ654–1339 | 1.7 | 1.0 | 134 |
| KK1463/4AA | 3.6 | 3.0 | 183 |
| R1496A | 4.3 | 2.2 | 322 |
| RR1479/80AA | 13.2 | 3.7 | 374 |
| K1522A | 2.2 | 1.6 | 61 |
| MARCKS | 1.2 | 1.1 | 105 |
| DdUnc104PH | 0.9 | 1.2 | 69 |
Average worm velocities are expressed in mm/min ± SD with the number of worms analyzed (n). In comparison, N2 wild-type worms move at a velocity of 13.4 ± 4.5 mm/min (n = 85). Numbers are derived from at least two independent experiments. The velocities of ΔPH, MARCKS. DdUnc104PH, was not statistically different from the unc-104 animals (p > 0.001), but the other constructs were statistically different (p < 0.001).