Figure 5. RSF1-deficient cells suffering from chromosome-alignment defects are rescued by RSF1 that retains the ability to bind PLK1.

(a) After RSF1 shRNA cells stably expressing the RSF1 shRNA plasmid were co-transfected with H2B-GFP and indicated RSF1-V5 constructs, cells were synchronized at the G₁/S boundary by adding 2 mM of thymidine for 24 h and released from the thymidine block for 8 h. Time-lapse images were acquired every 5 min. Scale bar, 5 μm. (b) Quantification of aligned or unaligned chromosome (%) in the acquired images in a. At least 30 cells on average were traced in three independent experiments (n=30). Data are represented as means±s.e.m. (n=3). **P<0.01, ***P<0.001 by Student's t-test. (c) A proposed model for recruitment of PLK1 to the kinetochore by RSF1.