Figure 3. Gene-silencing effect with Toc-HDO targeting scavenger receptor class B member 1 (Srb1) in the liver.

(a) qRT–PCR analysis showing Srb1 mRNA levels in liver assayed 3 d after injection of ASO or Toc-HDO-targeting Srb1, shuffle sequence of Toc-HDO-targeting Srb1 or PBS alone. Data are expressed as mean values±s.e.m. (n=3, **P<0.01 versus PBS). (b) Western blot analysis of Srb1 protein in livers from three mice 7 d after injection of 6 mg kg⁻¹ Toc-HDO-targeting Srb1 mRNA. (c) qRT–PCR analyses showing Srb1 mRNA levels in liver assayed 3 d after injection of DNA/2′-MOE gapmer ASO, Toc-HDO or PBS alone. Data are expressed as mean values±s.e.m. (n=4, **P<0.01 versus PBS). (d) qRT–PCR analyses showing Srb1 mRNA levels in liver assayed 3 d after injection of mice with DNA/cEt gapmer ASO, Toc-HDO or PBS alone. Data are expressed as mean values±s.e.m. (n=4, **P<0.01 versus PBS). Data are representative of at least three independent experiments each (a,c,d). P values were calculated from the Student's two-tailed t-test (a,c,d).