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. 2015 Aug 10;6:7969. doi: 10.1038/ncomms8969

Figure 7. Incorporation of Toc-HDO into lipoproteins.

Figure 7

(a) Diffusion time of Cy3-labelled oligonucleotides in HDL fraction of serum (ex vivo) or the serum extracted from mice 10 min after injection of 3 mg kg−1 Cy3-labelled ASO or Toc-HDO (in vivo) determined by fluorescence correlation spectroscopic analysis (n=10, mean values±s.e.m.). HDL or LDL fraction (control) was stained using Nile Red. (b) Gel-shift assay of Toc-HDO with extracted mouse HDL or LDL. (c) Oligonucleotide concentration of serum lipoprotein fractions 30 min after injection of 6 mg kg−1 AlexaFlour 647-labelled Toc-HDO (n=3, mean values±s.e.m.). ND, not detected. (d) qRT–PCR analyses of ApoB mRNA levels in liver 3 d after injection of 0.75 mg kg−1 Toc-HDO into wild-type or Ldlr−/− mice. Data are expressed as mean values±s.e.m. (n=3, **P<0.01). Data are representative of at least three independent experiments each (a,c,d). P values were calculated from Student's two-tailed t-test (d).