Figure 3.

Zinc-knuckle motif of hSlu7 binds zinc. The amount of zinc released from recombinant wild-type hSlu7 (hSlu7-WT) and a zinc-knuckle mutant version of the same protein (hSlu7-mut) was measured using an ICP-AES. The figures are an average of three measurements. H₂O, the metal-free water we used for our samples; buffer only, a mock sample with no protein; and hSlu7-WT, purified recombinant wild-type hSlu7; hSlu7-mut, purified recombinant zinc-knuckle mutant hSlu7 (SSGS mutant; see text). SD is in ppb.