Table 2.
Oligonucleotides used in this study
| Oligonucleotide, no. | Sequence (5′ → 3′) | Used for |
|---|---|---|
| Plasmid constructiona,b | ||
| 556 | GTACTAGTCGACcagttatttgtacaattcatcc | pUG24 |
| 557 | CCACTAGTCGACatgtctaaaggtgaagaattattc | pUG24 |
| 1098 | CCACTAGAGCTCTCTCCACATACCAATCACTCG | pUG64 |
| 1099 | GTACTAGTCGACTGGTCTTTGCATAGTGTGCG | pUG64 |
| 327 | GCCGTAAGACTAGTATGATAAATTGCAAGGTC | pUG15 |
| 328 | GGCGCCGGGTCGACTTATTCCAAACCTTTATAAAT | pUG15 |
| 345 | CGCACGTGACGTCTTATTCCAAACCTTTATAAA | pSH50 |
| 346 | CGCATGCCATGGTAAATTGCAAGGTC | pSH50 |
| 327 | GCCGTAAGACTAGTATGATAAATTGCAAGGTC | pUG38 |
| 391 | ACTAAGAATTCTTCCAAACCTTTATAAAT | pUG38 |
| Gene disruptionb | ||
| 278 | TTTGAAATATTCTCTAAATTATGCAGTTATTTCTCGTGATcagctgaagcttcgtacgc | FOL1 |
| 279 | TACAAAACAGAATTACATATTTAATCTATATAACTAACCTgcataggccactagtggatctg | FOL1 |
| 1052 | ATGAAAGTCCAAATAACCAATAGTAGAACAGAGGAAATCTcagctgaagcttcgtacgc | STE12 |
| 1053 | ATATCAGGTTGCATCTGGAAGGTTTTTATCGGACCTTCGAgcataggccactagtggatctg | STE12 |
| 1056 | ATGCAAAGACCATTTCTACTCGCTTATTTGGTCCTTTCGCcagctgaagcttcgtacgc | FLO11 |
| 1057 | GTATCATTAGAATACAACTGGAAGAGCGAGTAGCAACCACgcataggccactagtggatctg | FLO11 |
| 591 | ACCCAACTGCACAGAACAAAAACCTGCAGGAAACGAAGATAAATCatgtctaaaggtgaagaat | URA3 |
| 592 | AATTTGTGAGTTTAGTATACATGCATTTACTTATAATACAGTTTTgcataggccactagtgga | URA3 |
| 1425 | CTGGAATTCATAATTCATTAAGCAGAAAATATGTACCATGcagctgaagcttcgtacgc | PHD1 |
| 1426 | TTGTTCATAGAGCAAAGAGTTAACGGATTATGTTATGTGCgcataggccactagtggatc | PHD1 |
| 1427 | ATAATCCACCTATTTCAACAATTCTGATACCTGTTTAACCcagctgaagcttcgtacgc | TEC1 |
| 1428 | TGCGTATTTATGTACGAGATGTATGTATGTATGTAGACATgcataggccactagtggatc | TEC1 |
| Tagging | ||
| 609 | TTTCCTCCCTTGGTTATTTTTAACGATTCTTTATTATGAAgcataggccactagtggatctg | FOL1 |
| 636 | GAGCATTAAATTAGCAGATGCTATTTATAAAGGTTTGGAAatgtctaaaggtgaagaattattcac | FOL1 |
| Verification | ||
| 363 | GGATGTATGGGCTAAATG | kanMX forw |
| 335 | CCTCGACATCATCTGCCC | kanMX rev |
| 1068 | TTTGGGATCCCTCCCGTG | YNL256w start |
| 1069 | TTGTACCATGCCAAGAAC | YNL256w stop |
| 638 | ATAGATGAAGTTTTCGTGTG | STE12 start |
| 1055 | GAAAGATGTATCGGTAGC | STE12 stop |
| 1058 | CGTTCTCTTCTGATGAGG | FLO11 start |
| 1061 | TTCATCAAAGCCTGGTCG | FLO11 stop |
| 604 | GCATCACCTTCACCTTCACC | yEGFP3 |
| S52 | GTTTCAAACACATTCAAATGG | FOL1 |
| S38 | GGAACAATCACTTATCATGC | FOL1 |
| 593 | GAGGCTACTGCGCCAATTG | URA3 |
| 604 | GCATCACCTTCACCTTCACC | URA3 |
| 1429 | GCTGGAGTTTCTCTCGATGG | TEC1 |
| 1430 | TACTCGTTCTGGCGCGTCAG | TEC1 |
| 1431 | ATATAGGAAGAACTCTACAG | PHD1 |
| 1432 | TCTAACGAATTGCGCAATAG | PHD1 |
a
Underlines indicate restriction enzyme recognition sites
b
Lower case letters indicate nucleotides homologous to sequences on plasmids used for PCR; capital letters indicate nucleotides homologous to genomic sequences in yeast used for homologous recombination