Table 4.

Increased bipolar budding pattern in haploid fol1 deletion strains

Strain	Unipolar (%)	Random (%)	Bipolar (%)
wt	30	23	47
fol1Δ	5	4	91
fol1Δ ste12Δ	6	21	73
fol1Δ fol1Δ	6	7	87
fol1Δ phd1Δ	4	8	88
fol1Δ tec1Δ	11	5	84

Yeast cells were grown for 24 hrs on YPD plates supplemented with 50 μg/ml folinic acid, then analyzed. For the determination of budding pattern of cells having two or more bud scars, the following criteria applied: unipolar: cells with all bud scars at one pole; bipolar: cells with bud scars at both poles; random: cells with at least one bud scar not localized at either pole (Freedman, et al.; 2000). Values are shown as percentages (n ≥ 100 cells).