Table 5.
FLO11 promoter activity in different strains
Strain | RGF |
---|---|
wt | 21.91 ± 3.36 |
ste12Δ | 17.15 ± 5.04 |
fol1Δ | 104.87 ± 18.68 |
ste12Δfol1Δ | 24.11 ± 6.16 |
Yeast strains expressing yEGFP from the FLO11 promoter present on a plasmid were grown in liquid synthetic glucose media supplemented with 250 μg/ml folinic acid to an OD600 of 0.3–0.5. The amount of yEGFP protein was quantified by flow cytometry. For each data point, five independent cultures were measured. Median ± SD are given. RGF, relative green fluorescence.