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Searching for functional evidence of CYP-CPR complex formation: Initial conditions were- 20 μM DLPC, 40 or 50 μM 7EFC, 500 μM NADPH, 0–500 nM CPR or CYP1A2 as titrants. For the left panel, rates were calculated from slope of line obtained by the estimation of product at 3, 6, and 9 min, respectively. For the middle and right panel, reaction sampling was done at 12 and 10 min, respectively.
