Table 1.
Comparison of relative stoichiometries of a reconstituted and baculosome system.
| Rxn. | NADPH (nmoles/ml/min) | H2O2/NADPH (%) | Prdt./NADPH (%) | Water/NADPH (%) | H2O2/Prdt. | Water/Prdt. |
|---|---|---|---|---|---|---|
| Baculosome | 0.191 ± 0.01 | 43*, 0 | 57, 50 | 0, 50 | 0.75, 0 | 0, 1 |
| Reconstituted | 1.936 ± 0.05 | 61, 25 | 0.2, 0.1 | 39, 75 | 339, 278 | 217, 833 |
| Autocatalysis | 0.070 ± 0.02 | 100*, 0 | ~0, ~0 | 0, 100 | ∞, ∞ | ∞, ∞ |
The absolute peroxide concentrations in baculosome, reconstituted and autocatalytic systems were (2.1, 0), (17.7, 28.9) and (1.1 and 0) nmoles/ml, respectively at 15 and 60 min. Specific hydroxylated product formed was (1.66, 5.71) and (0.053, 0.11) nmoles/ml for baculosome and reconstituted systems, respectively at 15 and 60 min. Calculation of water formation was: NADPH consumed − (product formed + peroxide formed). Initial conditions are given in the legend to Figure 1. (
*
slight overshoot in peroxide estimation is noted).