Table 4.

Simulation of hydroxylating activity of CYP2C9 with DROS.

Reaction	Without CYP2C9	With CYP2C9
CPR+NADPH	Trace	911
H2O2	<10	~41
O2·−	~112*	~995*

Concentrations of specific 4'hydroxylated diclofenac formed after 10 min incubation (followed by termination of the reaction with the stopping agent) are given in nM. Initial concentrations of the reaction components were- [Diclof] = 25 μM, [DLPC] = 20 μM, [CYP2C9] = 100 nM. When present, the initial concentration of the following components was—[NADPH] = 200 μM, [CPR] = 200 nM, [H₂O₂] = 32 μM, [KO₂] ≤ 16 μM.

^*Non-specific hydroxylations or side reactions were noted.