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. 2004 Aug;15(8):3863–3875. doi: 10.1091/mbc.E03-08-0584

Figure 6.

Figure 6.

Smad1/5 phosphorylation induced by BMP6 and BMP2 after infection of AHPs with adenovirus carrying the sequence for dnAlk3 or dnAlk6. (a) Cells were infected with adenovirus carrying the sequence for LacZ or dnAlk3 (moi 300) at DIV2. At DIV4, different concentrations of BMP6 were added, and cell lysates were collected 3 h later. Eighty micrograms of protein from whole cell lysates was separated by SDS-PAGE and blotted with anti-phospho-Smad1/5. (b–d) Cells were infected with different amount of adenovirus (b, moi 50; c, moi 200; and d, moi 400) carrying the sequence for LacZ, dnAlk3, or dnAlk6 at DIV2. At DIV4, 50 ng/ml BMP6 or BMP2 was added, and cell lysates were collected 3 h later and processed as described for a.