Figure 3.

The effect of substitutions at sites 83 and 292 on spectral tuning and rates of retinal release in great bowerbird (A), chicken (B), and bovine (C) rhodopsin. N83 slows down retinal release and blue‐shifts λ _max while D83 accelerates retinal release and red‐shifts λ _max relative to all three wild‐type conditions. S292 accelerates retinal release and greatly blue‐shifts λ _max relative to wild‐type conditions in both great bowerbird and bovine rhodopsin. The indicated λ _max values were estimated according to the curve‐fitting methodology of Govardovskii,72 and half‐lives were estimated by fitting time courses to first‐order exponential curves.