Table 1. Cultured isolates from University Valley.
| Isolate | Environment of closest BLAST match | % Similarity | Temp. growth range (°C)a | Salinity growth range (% NaCl) | Isolation media | Enrichment step before isolation | Sample | GenBank accession number |
|---|---|---|---|---|---|---|---|---|
| Rhodococcus sp. (Bacteria) | Cloud water (1465 m), France (HQ256820.1) | 97% | −5–30 | 0– 7 | R2A, 5 °C | 0.1% Na4P2O7, 2 weeks | IT-12, 37–42 cm | KM279631 |
| Methylobacterium sp. (Bacteria) | Moss phyllosphere (NR_117561.1) | 96% | 5– 30 | 0–5 | TSA+5% NaCl | TSA+5% NaCl, 2 weeks | IT-5, 5–10 cm | KM279632 |
| Rhodotorula sp. (Fungi) | Lake Vostok accretion ice, Antarctica (EU108797.1) | 99% | −10–30 | 0–15 | R2A, 5 °C | 0.1% Na4P2O7, 2 weeks | IT-12, 37–42 cm | KM279633 |
| Sphingomonas sp. (Bacteria) | Surface Soil, South Korea (NR_043171.1) | 95% | 5–25 | 0–5 | R2A | TSA+5% NaCl, 2 weeks | IT-12, 12–15 cm | KM279634 |
| Unidentified Chaetothyrialesb (Fungi) | University Valley sandstone endolith collected 1980/81 (GU250317.1) | 99% | 0–25 | ND | 1:10 TSA, 5 °C, 50 days; 1:2 R2A, TSA, 5 °C, 40 days | No enrichment | IT-12, surface soil; IT-5, 5–10 cm | KM279635 |
| Bacillus sp. (Bacteria) | Taibai mountain, China (KJ589539) | 99% | ND–25 | ND | R2A, anaerobic conditions | Perchlorate-reducing media | IT-5, 5–10 cm | KM279636 |
Abbreviations: ND, Not determined; Temp, temperature; TSA, tryptone soy agar.
a
No strains were found to grow at 37 °C.
b
Cannot identify to the genus level.