Table 2.
Sedimentation analysis of mutants with a clumping phenotype
| Sedimentation timeb
|
|||
|---|---|---|---|
| Genotypea | Strain | 80% of initial OD595 | 50% of initial OD595 |
| Wild type | 972 | >30 | >30 |
| ace2Δ | ND064 | 6 ± 1 | 9 ± 1 |
| sep1Δ | ND102 | 5 ± 1 | 10 ± 2 |
| ace2Δsep1Δ | ND148 | 4 ± 1 | 6 ± 1 |
| agn1Δ | ND001 | 17 ± 2 | 21 ± 2 |
| eng1Δ | ND005 | 19 ± 1 | 22 ± 1 |
| agn1Δeng1Δ | ND019 | 11 ± 1 | 14 ± 1 |
| agn1Δ [pND02] (ON) | ND234 | 30 ± 5 | >30 |
| agn1Δ [pND02] (OFF) | ND234 | 19 ± 1 | 26 ± 1 |
| agn1Δ [pSE01] (OFF) | ND235 | 16 ± 1 | 20 ± 1 |
| agn2Δ | ND028 | >30 | >30 |
a
Complete genotypes are shown in Table 1
b
Sedimentation times (minutes) are the times required for the OD595 of the individual cultures to decrease to 80% or 50% of their initial values and are the mean ± SD of four individual experiments, except those of strains with genotypes wild type (n = 12), agn1Δ (n = 8), and ace2Δ (n = 8)