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. Author manuscript; available in PMC: 2017 Jun 20.
Published in final edited form as: Dev Cell. 2016 Jun 20;37(6):558–570. doi: 10.1016/j.devcel.2016.05.015

Figure 5. Inhibition of autophagy leads to lipid accumulation, which is coupled to nutrition.

Figure 5

(A) Lipid droplets accumulate upon RNAi mediated depletion of Atg1, Atg7, Atg8a in the PG and in the PG of Atg8aKG07569 mutants, quantified in (B). (C) Ex vivo incubation assay shows that NBD-cholesterol (green) co-localize with mCherry-Atg8 positive vesicles (red) in PG cells. mRNA levels (D) and protein levels (E) of genes involved in cholesterol uptake and trafficking in L3 larvae fed on normal food versus L3 larvae starved for 10 hours. Detailed description of genotypes is given in Supplemental Experimental Procedures. Error bars indicate SEM (*P<0.05; **P<0.01; ***P<0.001).