Figure 6.

Levels of talin protein are lower in phg2 and myoVII null cells. Cell lysates from wild-type or mutant cells were separated on an SDS-polyacrylamide gel (10⁵ cells/lane), transferred to a nitrocellulose membrane, and the amount of talin (top gel), p80 (medium), and Phg1 (bottom gel) was assessed by Western blot. Whereas the amounts of Phg1 or p80 protein (an endosomal marker) were not affected, the amount of talin was reduced in phg2 and myoVII mutant cells. This experiment was repeated three times with identical results.