Figure 8.

Polymerization of actin in focal sites is affected in phg2 mutant cells. Wild-type (WT) and mutant cells were allowed to adhere to a glass coverslip for either 2 or 10 min in phosphate buffer, and then fixed and stained with phalloidin. The actin cytoskeleton just above the plane of contact with the coverslip was imaged by confocal microscopy as described in Figure 7 (CS). After 10 min, unusually large accumulations of actin were seen in phg2 mutant cells. Confocal images taken within the cell body (CB) showed that the actin staining was essentially identical in all four mutants compared with wild-type (our unpublished data). Bar, 10 μm.