Figure 1.

Schematic representation of the ADMSC co-culture system in the presence of a SF/CBPG scaffold.

A 12-well co-culture system (Nunc) was used. ADMSC were placed on the bottom layer and cultured in DMEM plus 0.2% BSA. In the upper layer we placed the SF/CBPG scaffold (column A), CBPG (column B) and SF (column C). A control in which ADMSC were cultured in standard conditions (DMEM +10% FBS) was also prepared (column D). After the first 4 days we observed a reduction of cell growth and the formation of 3D multicellular spheroids both in cells cultured with the SF/CBPG scaffold and CBPG. The analysis was repeated three times on six different ADMSC lines.

ADMSC: adipose mesenchymal stem cells; SF/CBPG: silk fibroin/cord blood platelet gel; DMEM: standard culture medium.