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. 2016 May 6;14(1):57–68. doi: 10.3892/mmr.2016.5215

Figure 6.

Figure 6

Effects of melittin on the proportion of CD34/CD133 double-positive cells in the mouse model of UMR 106 osteosarcoma. Intra-tumor multipoint local injections were administered when the tumors grew to ~0.5×0.5 cm. For the NC group, tumors were treated with a local injection of normal saline (total volume, 200 µl) once a day. Tumors were treated with local injections of 160, 320 or 640 µg/kg melittin, once a day, for a treatment period of 5 days. Mice were treated for two 5-day periods, with one day between the two periods. CD34 (green, FITC) and CD133 (red, Cy3) expression in the tumor tissue samples was detected using fluorescence microscopy (magnification, ×100). The proportions of CD34/CD133 double-positive cells are presented as means ± standard deviation (n=6/group). **P<0.01, ***P<0.001 vs. NC; #P<0.05, ##P<0.01 vs. 160 µg/kg melittin; △P<0.05 vs. 320 µg/kg melittin. CD, cluster of differentiation; NC, negative control; FITC, fluorescein isothiocyanate; Cy3, cyanine 3.