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. 2016 May 24;7(6):2362–2372. doi: 10.1364/BOE.7.002362

Fig. 3.

Fig. 3

OPO is more efficient than NLO to excite QD655 in the healthy mouse spinal cord. (A). Images taken at various depths (140 to 240µm below the dura-mater) under an 800nm NLO or 1050nm OPO excitation in the same region of interest in the spinal cord of a living mouse intravenously injected with a solution of QD655. Arrowheads highlight the improvement of XY spatial resolution under an OPO excitation (B). XY and orthogonal (Z) reconstruction of the central vein at 200µm below the dura-mater under an 800nm NLO or 1050nm OPO excitation. Arrows: diving blood vessel highlighted in the bottom panel. Bottom panel: Y profiles of a diving vein at various depths demonstrate that OPO excitation enables observations at greater depths than NLO. Scale-bar: 100µm.