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. 2016 May 24;7(6):2362–2372. doi: 10.1364/BOE.7.002362

Fig. 4.

Fig. 4

QD655 can be used in combination with fluorescent proteins. (A-B). DsRed2 (green) and QD655 (red) emission spectra under an 800nm NLO (A) or 1050nm OPO (B) excitation. (C). Spectrally unmixed image of GL261 DsRed2-expressing cells (green) growing on a glass coverslip with QD655 (red) added in the medium using the spectral deconvolution algorithm of the Zeiss Zen 2012 software. Scale-bar: 25µm. (D). Emission intensity of DsRed2 expressed in GL261 cells under an 800nm NLO (green) or 1050nm OPO (red) excitation. Emission was normalized at 100 for NLO excitation. *: p = 0.002, Wilcoxon signed rank test. (E). XY and orthogonal (Z) reconstruction of the central vein of the spinal cord at 180µm below the dura-mater under a 940nm NLO or 1050nm OPO excitation in a CD11c-EYFP mouse. Dotted line: CD11c-EYFP+ cell. Scale-bar: 100µm. (F). Tridimensional reconstruction of an area of the cerebral cortex of a Thy1-CFP/CD11c-EYFP mouse from a sequential 800nm NLO and 1050nm OPO intravital acquisition. Green: Thy1-CFP neurons (NLO), Red: QD655 in blood vessels (OPO), Magenta: CD11c-EYFP dendritic cells/microglia (OPO). (G). Tridimensional reconstruction of the upper part of the spinal cord of a Thy1-CFP/LysM-EGFP/CD11c-EYFP mouse from a sequential 800nm NLO and 1050nm OPO intravital acquisition. Cyan: Thy1-CFP neurons (NLO), Green: LysM-EGFP monocytes (NLO), Yellow: CD11c-EYFP dendritic cells/microglia (OPO), Red: QD655 in blood vessels (OPO).