Table III.
Cell percentage following DHEA-treatment.
| Group | Cell cycle stage
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 6 h
|
12 h
|
24 h
|
48 h
|
|||||||||
| G0/G1 (%) | S (%) | G2/M (%) | G0/G1 (%) | S (%) | G2/M (%) | G0/G1 (%) | S (%) | G2/M (%) | G0/G1 (%) | S (%) | G2/M (%) | |
| Vehicle | 56.61±1.89 | 27.61±0.37 | 15.78±2.265 | 35.98±1.38 | 38.55±1.78 | 25.46±0.48 | 58.96±0.96 | 23.30±0.70 | 17.75±0.26 | 69.16±2.36 | 21.55±2.03 | 9.29±0.77 |
| 1 µmol/l DHEA | 55.76±2.98 | 30.91±4.42 | 13.23±1.63 | 36.10±1.34 | 37.19±2.27 | 27.72±3.35 | 54.01±1.01 | 25.79±2.49 | 20.21±1.47 | 66.82±0.84 | 24.82±2.31 | 8.36±0.61 |
| 50 µmol/l DHEA | 57.62±1.23 | 30.41±1.45 | 11.97±0.24 | 35.18±1.57 | 40.29±1.51 | 24.54±0.15 | 58.10±2.04 | 27.88±1.62 | 14.03±1.24 | 68.29±0.72 | 26.98±0.53b | 4.73±0.66b |
| 100 µmol/l DHEA | 56.20±1.58 | 31.66±1.39 | 11.99±0.47 | 36.71±1.84 | 48.34±0.99b | 14.95±1.72b | 55.60±3.86 | 38.86±4.02b | 5.55±1.03b | 67.12±0.50 | 31.10±1.11b | 1.78±0.29b |
Cell cycle distribution was calculated from 10,000 cells with ModFit LTTM software using FACScaliber. Values are presented as the mean ± standard error (n=12).
a
P<0.05,
b
P<0.01 vs. vehicle group. DHEA, dehydroepiandrosterone.