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. 2016 May 10;14(1):218–224. doi: 10.3892/mmr.2016.5239

Figure 2.

Figure 2

Cyclic mechanical stretch activates cofilin in osteoblast-like MG-63 cells. (A) Western blot analyses and quantification of stretch-induced protein expression of (B) cofilin and (C) p-cofilin in MG-63 cells under cyclic stretch (n=6). (D) Evaluation of the mRNA expression levels of cofilin under cyclic stretch via reverse transcription-quantitative polymerase chain reaction analysis (n=6). Data are presented as the mean ± standard deviation. *P<0.05, **P<0.01 vs. control; ##P<0.05 vs. 4 h. (E) Immunofluorescence analyses for stretch-induced protein expression of cofilin (scale bar = 50 µm). p-cofilin, phosphorylated cofilin; Ctrl, control; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; DAPI, 4′,6-diamidino-2-phenylindole.